FIG. 1.

Accumulation of pp71 protein in cells infected with recombinant adenovirus containing the UL82 gene compared to CMV-infected cells. Shown is a film image of electrophoretically separated cell lysates reacted with antibody to pp71. Cultures of U373:CIITA cells in six-well plates were either mock infected or exposed to 30 PFU/cell of the AD169 strain of human cytomegalovirus or 100 PFU/cell of Adβgal or Adpp71. Cells were harvested 24 h and 72 h after exposure to virus and solubilized, and 30 μg of protein from each lysate was subjected to electrophoresis in a denaturing polyacrylamide gel. Proteins were transferred to nitrocellulose sheets and reacted with mouse monoclonal antibody to pp71 and, as an additional loading control, mouse monoclonal antibody to GAPDH, as described in Materials and Methods.