FIG. 2.

Accumulation of cell surface molecules in cells infected with CMV or recombinant adenoviruses expressing β galactosidase, or pp71. Shown are cell surface MHC class I and CD71 levels in cells expressing pp71. Cultures of U373:CIITA cells in 24-well plates were either mock infected or exposed to 15 or 30 PFU/cell of the AD169 strain of human cytomegalovirus or 100 PFU/cell of defective adenovirus Adβgal or Adpp71. Cells were dislodged from the plates at the indicated times after infection, reacted with FITC-conjugated anti-MHC class I (A) and PE-conjugated anti-CD71 antibodies (B), and analyzed by flow cytometry. Data points represent least-square mean fluorescence intensities and standard errors of the means for between 6 and 12 replicate samples for each infection condition at each time point, which were obtained from three independent experiments.