FIG. 3.

Effects of pp71 on accumulation cell surface MHC class I cannot be attributed to nonspecific effects of exposure to recombinant adenoviruses. (A) MHC class I cell surface levels in cells exposed to increasing doses of defective recombinant adenoviruses. Cultures of U373:CIITA cells in 24-well plates were exposed to defective adenovirus Adβgal or Adpp71 at increasing multiplicities of infection. Cells were dislodged from the plates 48 h after infection, reacted with FITC-conjugated anti-MHC class I antibodies, and analyzed by flow cytometry. Shown are least-square mean fluorescence values and standard errors of means from five replicate samples derived from two independent experiments. (B) Histogram of MHC class I cell surface levels in transfected cells. Cultures of U373 cells in six-well plates were mock transfected or transfected with plasmid pcDNA3.1 containing the UL82 gene or plasmid pEGFPN3. Histograms are shown for cells reacted with FITC-conjugated anti-MHC class I antibodies compared to unstained cells.