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Bax-deficient HCT116 cells are selectively resistant to THG. (A) HCT116 Bax+/− and Bax−/− cells were treated with 1 μM THG or 1 μg/ml BFA for the indicated periods of time and subjected to trypan blue dye exclusion assay. (B) HCT116 Bax+/− and Bax−/− cells were treated with 1 μM THG or 1 μg/ml BFA for the indicated periods of time, and 1 × 104 cells were reseeded on 100-mm plates in normal growth medium. Ten days later, colonies were visualized with hematoxylin-eosin staining.
