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BFA activates caspase 3 independently of Bax while THG generates high-molecular-mass species of caspase 3. HCT116 Bax+/− or Bax−/− cells were treated with 1 μM THG or 1 μg/ml BFA for the indicated periods and subjected to caspase 3 activity assay (A) or immunoblot analysis (B) with anti-caspase 3 rabbit antiserum or anti-α-tubulin monoclonal antibody. ΔFU, difference in fluorescence units.
