FIG. 5.

Summary of allowed and disallowed substitutions. Mutations that are highlighted in red impair reporter expression, whereas substitutions that are shown in blue are well tolerated. Azure indicates light-blue colonies and hence partial impairment. For residues in ordered portion of structure (residues 41 to 86), mutations at solvent-exposed sites are listed above the DM sequence, whereas those below the DM sequence indicate buried sites (side chain solvent accessibility, <40%). The polyalanine variant (residues 98 to 105) is indicated by a black bar.