FIG. 6.

Stop codon readthrough mediated by E. octocarinatus/S. cerevisiae (Eo/Sc) eRF1 measured after depletion of S. cerevisiae (Sc) eRF1. (A) Western blot confirming the relative levels of eRF1 proteins. (Top) Blot probed with polyclonal antibodies to domains 2 and 3 of yeast eRF1 that should recognize all forms of eRF1; (bottom) blot probed with antibodies to the HA epitope tag. Cultures were grown in SM medium with either glucose or galactose as the carbon source to regulate the expression of S. cerevisiae eRF1 under GAL1 promoter control. (B) Stop codon readthrough measured in cells expressing S. cerevisiae eRF1, no eRF1, or E. octocarinatus/S. cerevisiae eRF1. Cultures were grown at 30°C.