FIG.1.

Expression patterns of Hmgn1 during mouse embryogenesis. (A) Whole-mount in situ hybridization with digoxigenen-labeled Hmgn1 antisense probes. At E7.5 strong expression is observed in the embryonic region (arrowhead). No signal is observed in the ectoplacental cone (arrow). At E8.5 and E9.5, strong expression of Hmgn1 is visible throughout the embryo except in the heart (arrowhead). At E10.5, HMGN1 is most strongly expressed in the distal portion of forelimb and hind limb mesenchyme (filled arrowhead), in branchial arches (arrow), and in tail bud (open arrowhead). After stage E11.5 Hmgn1 expression remains strong mostly in the developing forelimbs and hind limbs and in the tail. As the embryos develops, Hmgn1 becomes progressively more localized to the interdigit mesenchyme of the limbs and to the tail. (B) Whole-mount in situ hybridization with Hmgn1 sense probes. (C) Bar graph depicting the relative expression levels of Hmgn1 transcripts at the developmental stage indicated or in the tissue indicated, determined by quantitative dot blot analysis with RNA master blots (Clonetech), relative to 7-day embryos, which are considered as 100%.