FIG. 3.
Suppression of rec2 radiation sensitivity and restoration of Rad51 focus formation. (A) rec2 strains (UCM54) expressing Rad51, Brh2, Brh2-RPA70, RPA70, or Rec2 were tested for survival after irradiation with UV (120 J/m2) or gamma rays (400 Gy). Serial 10-fold dilutions of cell suspensions were spotted from left to right as shown. wt, wild type. (B) rad51 (UCM628) or brh2 (UCM565) strains expressing untagged Rad51, Rec2, or Brh2 were tested for survival after irradiation with UV as described above. (C) GFP-Rad51 was expressed in rec2 strains with integrated transgenes expressing Brh2, Brh2-RPA70, or Rec2. Cells were monitored for Rad51 focus formation 60 min after administering a 40-Gy dose of gamma rays.