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. 2006 Jan;26(2):678–688. doi: 10.1128/MCB.26.2.678-688.2006

FIG. 5.

FIG. 5.

Meiotic rescue. Teliospores obtained from wild-type and rec2/rec2 homozygous crosses with or without an integrated transgene expressing Brh2-RPA70 were germinated on YEPS at 30°C for 38 h. (A) Teliospores (wild type) before germination viewed at ×1,000 magnification. (B) rec2/rec2 teliospores at 38 h after germination, arrested after promycelium formation. (C) rec2/rec2/Brh2-RPA70 microcolonies at 38 h. (D) Wild-type microcolonies at 38 h. Microcolonies from rec2/rec2/Brh2-RPA70 (E) and the wild type (F) were collected and dispersed to single cells which were spread onto medium containing charcoal to enable fuzz formation. Colonies appearing after incubation for 3 days were recorded. A section from petri dishes with Fuz+ (white) and Fuz− (gray) colonies is shown.