FIG. 6.

BS69 is specifically involved in the LMP1-meidated JNK pathway. (A) 293T cells were transfected with different shRNAs twice as indicated, followed by transfection of LMP1. WCEs were subjected to immunoblotting with both the anti-phospho-JNK and anti-total JNK antibodies. (B) 293T cells were transfected twice with pSuper or BS69 siRNA. Twenty-four hours after the last transfection, cells were treated with 20-ng/ml TNF-α for 10 min. WCEs were subjected to immunoblotting with anti-phospho-p38, anti-total-p38, anti-phospho-JNK, and anti-total JNK antibodies. Anti-β-tubulin was used here as a loading control. Endogenous JNK1 was also immunoprecipitated from WCEs and subjected to kinase assays (KA).