FIG. 7.

Fusion of BS69 to an LMP1 mutant without the cytoplasmic carboxyl terminus restores JNK activation. (A) 293T cells were cotransfected with HA-JNK, together with other expression vectors as indicated. HA-JNK was immunoprecipitated from cell lysates and subjected to in vitro kinase assays. Separately, a part of the cell lysates was also subjected to immunoblotting with various primary antibodies as indicated. (B) The key signal transducers in the LMP1-mediated JNK pathway and the flow of the signal are depicted in the cartoon. Although LMP1, BS69, and TRAF6 are each drawn as a trimer, we do not know for sure whether this is the case (except for TRAF6). We simply imply here that they all need to oligomerize to activate the JNK pathway.