FIG. 1.

Generation of TDAG8 KO mice. (A) Strategy for homologous recombination in the tdag8 locus. (B) Genotypic determination by Southern blot analysis of BamHI-digested tail genomic DNA hybridized with an external probe. (C) PCR analysis of tail genomic DNA using primers specific for the WT (+/+) allele (P1 and P2) and for the recombinant mutant allele (P3 and P4). (D) RT-PCR to demonstrate the absence of TDAG8 transcripts in KO mice and the expression of EGFP in TDAG8 heterozygous (+/−) and KO (−/−) mice.