FIG. 2.

Effects of DEX treatment on TDAG8 and EGFP expression. (A and B) In vitro and in vivo DEX treatment induced the upregulation of both the TDAG8 WT allele and the EGFP knockin mutant allele. Thymocytes from TDAG8^+/+ (WT) and TDAG8^+/− (HET) mice were treated in vitro with 10 nM DEX for 2 or 4 h, and the expression levels of TDAG8 and EGFP were analyzed at the RNA level by RT-PCR. (B) Northern blot analysis of TDAG8 and EGFP expression following in vivo DEX treatment. Mice were injected i.p. with 100 μg of DEX diluted in PBS and euthanized 20 h later. Predicted sizes for the WT and KO transcripts were 2.036 kb and 3.091 kb, respectively. (C) Upregulation of the EGFP signal in thymi from TDAG8 KO mice following in vivo DEX treatment. Images of thymi explanted 20 h after DEX injections (100 μg/mouse) were acquired using a Xenogen-IVIS cooled CCD optical system, as described in Materials and Methods.