FIG. 4.

Normal immune development in TDAG8 KO mice. (A) Equivalent distributions of DN, DP, and single-positive CD4⁺ and CD8⁺ populations in thymi from WT and KO mice. (B) Comparison of total cell numbers from thymi explanted from 4- to 8-week-old WT (n = 31) and KO (n = 24) mice. (C) Compositions of B220⁺ and CD3⁺ lymphoid populations from WT and KO spleens. (D) Comparison of total cell numbers from spleens explanted from 8- to 16-week-old WT (n = 36) and KO (n = 31) mice. (E) Strategy to examine the positive and negative selection of WT and KO DO11.10 TCR-transgenic splenocytes. These cells are positively selected on the BALB/c strain expressing the I-A^d MHC class II haplotype. In contrast, the DO11.10 cells are negatively selected on the C57BL6 × BALB/c F₁ strain expressing both I-A^d and I-A^b MHC class II haplotypes. (F) Percentages of selected CD4⁺ DO11.10 TCR-transgenic T cells present in the spleens of mice on the BALB/c (n = 4 for each genotype) and C57BL6 × BALB/c F₁ genetic backgrounds (n = 4 for each genotype). DO11.10 TCR-transgenic CD4⁺ T cells were identified by FACS based on their reactivity with the anti-DO11 idiotypic antibody KJ1-26 (BD Pharmingen). The error bars indicate SEM.