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Characterization of OSR1 kinase activity. (A) Presence of autophosphorylated OSR1 at 77 and 60 kDa. (B) OSR1 autophosphorylation in the presence 2 and 5 mM MnCl2 and MgCl2. (C) Effect of increasing Cl− concentrations (4 to 40 mM) on 32P incorporation by the kinase. (D) OSR1 autophosphorylation as a function of ATP concentration. The Km was measured at ∼1 μM.
