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. 2006 Jan;26(2):472–479. doi: 10.1128/MCB.26.2.472-479.2006

FIG. 2.

FIG. 2.

Analysis of protein ISGylation and ubiquitination in Ube1L knockout cells. (A) Schematics of human ubiquitin-activating enzyme (UBE1) and ISG15-activating enzyme (UBE1L) indicating similar domain structures. ThiF, a repeated domain in ubiquitin-activating enzyme E1 and members of the bacterial ThiF/MoeB/HesA family; UBACT, a repeat in ubiquitin-activating enzyme E1. The numbers on the right side indicate the number of amino acids in each protein. (B) Bone marrow-derived macrophages from wild-type and Ube1L knockout mice were treated with 100 ng/ml of LPS for the time periods indicated in the figure. Cells were harvested and subjected to immunoblotting against ISG15 and ubiquitin.