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Ube1L-deficient cells are not hypersensitive to IFN-α/β-induced cell death. Bone marrow cells isolated from wild-type (WT) and Ube1L knockout (Ube1L KO) mice were cultured in vitro and treated with 100 U/ml IFN-β for 24 h and then treated with 1,000 U/ml IFN-β for an additional 48 h. An apoptosis assay was performed by staining cells with annexin V/7-AAD, followed by fluorescence-activated cell sorting analysis.
