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VSV protection assay. (A) Wild-type and Ube1L knockout MEFs were left untreated or treated with 100 or 1,000 U/ml of IFN-β for 24 h, followed by VSV infection ranging from a multiplicity of infection of 0 to 104 per well for an additional 24 h. Cell viability was assessed by crystal violet staining. (B) Ube1L+/+ (n = 5) and Ube1L−/− (n = 5) mice were infected intracerebrally with 1,000 PFU of LCMV and monitored daily for development of clinical symptoms and survival.
