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. 2006 Feb;80(3):1160–1166. doi: 10.1128/JVI.80.3.1160-1166.2006

TABLE 1.

Primers used for the amplification of Meq and Meq/vIL8

Primer Sequencea Template accession no.b
P1: SpeI/NheI-T7-Meq-For 5′-GGACTAGTATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGGA TGTCTCAGGAGCCAG-3′ AY243332 (RB1B Meq)
P2: Meq fusion-Rev (HindIII-XbaI) 5′-GCTCTAGAGCAAGCTTGCGGAATCCTCCGGGTCTCC-3′ AY243332 (RB1B Meq)
P3: Meq/vIL8 fusion-Rev (HindIII-XbaI) 5′-GCTCTAGAGCAAGCTTGCGGAATCCAAGACAGATATG-3′ AY243340 (Meq/vIL8)
a

Underlined nucleotides are restriction endonuclease sites introduced to facilitate cloning. Italicized nucleotides are encoded by the virus genome.

b

Accession numbers are from the NCBI nucleotide sequence database.