TABLE 1.
Primers used for the amplification of Meq and Meq/vIL8
| Primer | Sequencea | Template accession no.b |
|---|---|---|
| P1: SpeI/NheI-T7-Meq-For | 5′-GGACTAGTATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGGA TGTCTCAGGAGCCAG-3′ | AY243332 (RB1B Meq) |
| P2: Meq fusion-Rev (HindIII-XbaI) | 5′-GCTCTAGAGCAAGCTTGCGGAATCCTCCGGGTCTCC-3′ | AY243332 (RB1B Meq) |
| P3: Meq/vIL8 fusion-Rev (HindIII-XbaI) | 5′-GCTCTAGAGCAAGCTTGCGGAATCCAAGACAGATATG-3′ | AY243340 (Meq/vIL8) |
Underlined nucleotides are restriction endonuclease sites introduced to facilitate cloning. Italicized nucleotides are encoded by the virus genome.
Accession numbers are from the NCBI nucleotide sequence database.