FIG. 2.

Efficiency of DNA synthesis with WT and mutant enzymes. The levels of DNA synthesis were measured with the heteropolymeric primer/template substrate P20/T50 DNA as a function of time. FL denotes the full-length product, and P20 denotes the unextended primer. The asterisk points to the exonuclease activity of UL54 enzymes. This band is less pronounced in the control without enzyme (left, primer only) and in reaction mixtures that contain plasmid pCITE4b without the coding sequence for UL54 (right).