TABLE 2.
Inhibition of DNA synthesis in the presence of foscarnet and pyrophosphate
| Enzyme | Foscarnet
|
Pyrophosphate
|
||
|---|---|---|---|---|
| IC50 (μM)a | Fold resistanceb | IC50 (mM)a | Fold resistanceb | |
| WT | 0.47 ± 0.17 | 1 | 1.03 ± 0.22 | 1 |
| L802M | 1.68 ± 0.15 | 3.5 | 1.11 ± 0.02 | 1.1 |
| K805Q | 0.16 ± 0.05 | 0.3 | 0.62 ± 0.04 | 0.6 |
| Q807A | 2.98 ± 0.73 | 5.3 | 1.18 ± 0.23 | 1.1 |
a
IC50 values were determined in the presence of activated calf thymus DNA as described in Materials and Methods. Values are means of at least three independent determinations ± standard deviations.
b
Values of less than 1 represent hypersusceptibility.