Fusogenicity of S chimeras as determined by the cell-cell fusion assay. Fusion activity of the S chimeras was determined using the Renilla luciferase-based cell-cell fusion assay. Renilla activity of cell lysates was determined and corrected for the protein concentration. Subsequently, the background (S-independent rLuc activity) was subtracted and the remaining activity was plotted as a percentage of the wild type level. Columns: 1, Svsv-cyt; 2, Svsv-tmdcyt; 3, Smhv-tmdcyt; 4, Swt.