FIG. 3.

Restriction digests of RRV genomic DNA. DNA from purified RRV26-95 or cosmid-derived recombinant RRV-J, RRV-SEAP, and RRV-GFP was digested with either EcoRV (E), SphI (S), or the nicking endonucleases BbvCIA and BbvCIB (B). Restriction endonuclease-digested and undigested (U) virion DNA were separated on a 0.5% agarose electrophoretic gel and stained with ethidium bromide. Bands marked with an arrow represent altered digestion products resulting from insertion of the CMV-SEAP or CMV-GFP reporter gene cassettes. The molecular size standards (std) are HindIII-digested λ DNA.