FIG. 8.

SEAP expression by RRV-SEAP following neutralization by sera from rhesus monkeys naturally infected with RRV. RRV-SEAP (MOI of 0.006 PFU/cell) was incubated with either DH20 medium (No Antibody), sera from RRV-negative rhesus monkeys (Mm 288-94 and 320-98) or sera from rhesus monkeys naturally infected with RRV (Mm 140-83, 175-87, 180-91, 186-91, 186-92, 251-90, 295-00, 443-91, and 526-91). After a 3-h incubation at 37°C, rhesus fibroblasts were inoculated with either DH20 medium (No Virus) or the virus-sera mixture. At 24 h p.i., cultures were rinsed five times with HBSS and refed with DH20. At days 1 to 5 p.i., an aliquot of medium was removed to determine the level of SEAP expression and replaced with DH20 medium. The results show the average SEAP counts/s (CPS), with standard deviation, for each day p.i.