FIG. 4.

Features of F-MLV-transduced macrophages. (A) Transmission and fluorescence microscopy pictures of plated F-MLV-transduced macrophage cultures. (B) Cell surface marker analysis 2 days posttransduction of day 4 macrophages. Macrophages were incubated with the indicated fluorescently coupled antibody prior to flow cytometry analysis. (C) To demonstrate that viral transduction occurred in differentiated macrophages, day 4 macrophages were incubated with PE-labeled latex beads for 2 h followed by extensive cell washing to remove unbound beads. Two hours afterwards, the cell culture was infected. Cells were analyzed by fluorescence microscopy 3 days postinfection.