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Comparison between Moloney MLV and Friend MLV-derived retroviral vectors. The Friend MLV-derived vector was compared to a Moloney MLV-derived vector produced in parallel. This vector has been previously described elsewhere (23, 24), and it is comparable to the Friend MLV-derived vector used here. After normalization of their infectious titers on HeLa cells, equal MOIs were used in the transduction of day 4 macrophages, and infection was scored 3 days later by flow cytometry analysis.
