FIG. 6.

Absence of cell proliferation in human GM-CSF-derived macrophages. (A) Monocytes (gamma irradiated [γ-irr.] as a control or not, both with GM-CSF) and cycling Jurkat T cells were seeded in equal numbers in the presence of [³H]thymidine for 15 days prior to analysis. (B) Monocytes and PBLs were labeled with CFSE at day 0 and then differentiated with GM-CSF or stimulated with CD3 or CD28 plus IL-2 for 8 days, respectively, prior to flow cytometry analysis. The graph shows a comparison between CFSE fluorescence at day 0 and that at day 8, as indicated. (C) Day 4 GM-CSF-derived macrophages and Jurkat T cells were incubated for 24 h in BrdU, prior to staining with a fluorescently labeled anti-BrdU antibody and flow cytometry analysis. In each histogram plot, BrdU-negative and BrdU-positive samples (continuous and dotted lines, respectively) are superimposed.