FIG. 7.

Susceptibility of macrophages to retroviral transduction is time and culture condition dependent. (A) Monocytes were differentiated into macrophages in GM-CSF, M-CSF, or human serum (hAB) prior to viral infection at an MOI of 25 and flow cytometry analysis. (B) Time curve susceptibility to retroviral transduction of GM-CSF-derived macrophages. Time zero indicates cells immediately after the 3-h serum deprivation step and addition of GM-CSF and FCS. The results presented here represent the average of three independent experiments with cells of different donors.