FIG. 1.

ICP27 is associated with polyribosomes in infected cells. Cytoplasmic extracts of BHK cells either mock infected or infected with wt or M15 mutant virus were fractionated on 10% to 50% sucrose gradients, and fractions were examined by Western blotting using a mixture of anti-ICP27 1119 and 1113 monoclonal antibodies (Goodwin Institute for Cancer Research, Plantation, Fla.) or anti-PABP 10E10 antibody (9), kindly supplied by G. Dreyfuss. (A) UV absorbance profile (254 nm) of gradient fractions from mock-infected cells; the positions of polysomes, 80S monosomes, 60S and 40S ribosomal subunits, and mRNPs are indicated. (B) Western blot analysis of gradient fractions following mock infection or infection with the indicated viruses.