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Kinetics of self-processing and eIF4GI cleavage by CVB4 2Apro R20L. mRNA (∼10 ng/μl) from CVB4 VP1-2Apro R20L was used to program RRLs. Analysis of self-processing (A) and eIF4GI cleavage (B) were as described in the legend for Fig. 8. Protein standards in kDa are indicated at left of blots. cpN, N-terminal cleavage product; −RNA, without addition of mRNA.
