FIG. 6.

Results obtained with a replicon containing wild-type HCV sequence are consistent with those of cell culture-adapted replicons. Huh.8 cells were cultured under various conditions for 4 days before the total cell lysates were subjected to immunoblotting with anti-NS5A and anti-Ku86 antibodies. Lanes: 1, Huh-7; 2, Huh.8 untreated, nonconfluent cells cultured in 10% serum; 3, 1 μM 2CMA; 4, 10 μg/ml aphidicolin; 5, 0% serum; 6, Huh.8 cells cultured under confluence; 7, Huh.8 cells cultured under confluence but with uridine and cytidine (200 μM each) added to the culture medium.