TABLE 2.
Epitope mapping of anti-NS1 MAbs
| Antibody | Binding activity
|
|||||
|---|---|---|---|---|---|---|
| Yeasta
|
E. colib
|
|||||
| Full-length NS1 | FR-I | FR-II/-III | FR-III | FR-II/-III | FR-III | |
| 1NS1 | − | − | − | − | − | − |
| 2NS1 | − | − | − | − | ++ | ++ |
| 3NS1 | ++ | ++ | − | − | − | − |
| 4NS1 | ++ | − | ++ | ++ | ++ | ++ |
| 5NS1 | ++ | ++ | − | − | − | − |
| 6NS1 | ++ | − | + | + | ++ | ++ |
| 7NS1 | ++ | ++ | − | − | − | − |
| 8NS1 | ++ | ++ | − | − | ++ | − |
| 9NS1 | ++ | − | ++ | + | ++ | ++ |
| 10NS1 | ++ | ++ | ++ | − | ++ | − |
| 11NS1 | ++ | ++ | − | − | − | − |
| 12NS1 | ++ | ++ | − | − | − | − |
| 13NS1 | ++ | − | ++ | ++ | ++ | ++ |
| 14NS1 | ++ | − | ++ | + | ++ | ++ |
| 15NS1 | ++ | ++ | − | − | − | − |
| 16NS1 | ++ | ++ | − | − | − | − |
| 17NS1 | ++ | + | ++ | ++ | ++ | ++ |
| 18NS1 | + | − | + | + | ++ | ++ |
| 19NS1 | ++ | − | + | + | ++ | ++ |
| 21NS1 | ++ | − | − | − | − | − |
| 22NS1 | ++ | − | ++ | − | ++ | − |
| 23NS1 | ++ | ++ | − | − | − | − |
a
Yeast fragment binding was determined by flow cytometric analysis of binding to yeast expressing intact NS1 (NS1 [aa 1 to 352]), yeast FR-I (NS1 [aa 1 to 157]), yeast FR-II/-III (NS1 [aa 158 to 352]) or yeast FR-III (NS1 [aa 236 to 352]). Binding activity was scored as follows: −, <10%; +, 10 to 20%; ++, >20% positive compared to the negative control yeast transformed with the pYD1 vector.
b
Binding to E. coli-expressed NS1 fragments was determined by ELISA using E. coli FR-II/-III (NS1 [aa 125 to 352]) and E. coli FR-III (NS1 [aa 236 to 352]). Binding activity was scored as follows: −, <0.09; ++, >0.5 optical density at 450 nm.