TABLE 3.
Protective activity of anti-NS1 MAbsa
| Antibody or control | % Surviving mice | No. of mice | P value | Antibody source |
|---|---|---|---|---|
| PBS | 17 | 60 | ||
| 1NS1 | 25 | 20 | 0.29 | PUR |
| 2NS1 | 40 | 10 | 0.09 | ASC |
| 3NS1 | 50 | 10 | 0.02* | PUR |
| 4NS1 | 50 | 10 | 0.02* | PUR |
| 5NS1 | 40 | 10 | 0.10 | ASC |
| 6NS1 | 20 | 10 | 0.93 | ASC |
| 7NS1 | 0 | 10 | 0.66 | ASC |
| 8NS1 | 20 | 10 | 0.50 | ASC |
| 9NS1 | 40 | 10 | 0.15 | ASC |
| 10NS1 | 75 | 20 | 0.0001* | ASC/PUR |
| 11NS1 | 50 | 10 | 0.04* | ASC |
| 12NS1 | 10 | 10 | 0.95 | ASC |
| 13NS1 | 30 | 10 | 0.30 | ASC |
| 14NS1 | 95 | 40 | <0.0001* | ASC/PUR |
| 15NS1 | 0 | 10 | 0.74 | ASC |
| 16NS1 | 90 | 10 | 0.0001* | ASC |
| 17NS1 | 75 | 20 | <0.0001* | PUR |
| 18NS1 | 10 | 10 | 0.14 | ASC |
| 19NS1 | 20 | 10 | 0.36 | ASC |
| 21NS1 | 40 | 10 | 0.27 | ASC |
| 22NS1 | 70 | 10 | 0.004* | ASC |
| 23NS1 | 0 | 10 | 0.21 | ASC |
a
For in vivo protection studies with MAbs, 6-week-old C57BL/6 mice were inoculated with 102 PFU of WNV via the footpad and administered a single dose of purified MAbs (500 μg) or ascitic fluid (200 μl) at the same time through an intraperitoneal route. The mice were followed for 35 days. The data were obtained and P values were determined after comparison to the PBS-treated control group. Asterisks indicate statistical significance by the log rank test. ASC, ascites fluid; PUR, purified antibody; ASC/PUR, same result obtained when antibody was administered as either ascites or in a purified form in independent experiments.