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Mapping the VP19C NLS. BHK cells were transfected with plasmids expressing the GFP protein fused in frame to the N-terminal 83 (b), 76 (c), 66 (d), 56 (e), or 46 (f) amino acids of VP19C. (g and h) GFP is fused to amino acids 24 to 66 and 33 to 66 of VP19C. (a) GFP control. (i) N-terminal 70 amino acids of VP19C with the positions of various truncations indicated. Arginine residues are underlined.
