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Deletion of mutant N-ras in human fibrosarcoma cells restricts viral replication. Replicate cultures of HT1080 and MCH603 cells were infected with 1 PFU of R3616 or HSV-1(F) virus per cell in serum-free medium for 2 h, after which medium containing virus was removed and replaced with fresh medium containing 1% calf serum. At 36 h after infection, viral recovery was determined by standard plaque assay.
