FIG. 4.

Diminished [³⁵S]methionine metabolic labeling in virus-infected human fibrosarcoma cells deleted for mutant N-ras. Replicate cultures of HT1080 and MCH603 cells were infected with 10 PFU of R3616 or HSV-1(F) virus per cell. At 11 h after infection, the cells were rinsed, starved of methionine for 1 h, and then incubated in methionine-free medium supplemented with 100 μCi of [³⁵S]methionine per ml for two additional hours. At 14 h after infection, 20 μg of equilibrated protein lysates was electrophoretically separated in denaturing polyacrylamide gels, transferred to a PVDF membrane, and exposed to autoradiography film.