FIG. 5.

Increased PKR and eIF-2α phosphorylation in human fibrosarcoma cells deleted for mutant N-ras during R3616 infection. Replicate cultures of HT1080 and MCH603 cells were exposed to 10 PFU of R3616 or HSV-1(F) virus per cell. Cells were harvested at 14 h after infection and processed as described in Materials and Methods. The electrophoretically separated proteins were immunoblotted with antibodies for the phosphorylated form of PKR on threonine 446 and the phosphorylated form of eIF-2α on serine 51, as well as for total PKR and eIF-2α.