FIG. 7.

FLAG-MEK expression levels in HT1080 and MiaPaCa2 stable transfectants. Differences in cytopathic effects in virus infected caMEK and dnMEK stable cell lines are shown. (A) Equivalent amounts of whole-cell lysates from caMEK and dnMEK HT1080 and MiaPaCa2 stable transfectants were immunoblotted for FLAG-tagged caMEK and dnMEK using a monoclonal antibody to the FLAG-epitope. (B) Replicate cultures of HT-caMEK and HT-dnMEK cells were infected with 10 PFU of mock, R3616, or HSV-1(F) virus per cell. Photos were taken at 12 h after infection.