TABLE 1.
Sequence differences among E5, E5-104, and brain-passaged E5-104
| Gene | Nucleotide position (genome) | Nucleotide in indicated virus
|
Amino acid change | |||
|---|---|---|---|---|---|---|
| E5a | E5-104 | E5-104 isolate from mouse brainc
|
||||
| Isolate 1 | Isolate 2 | |||||
| E | 1306 | C | U | U | U | None |
| 1325 | G | U | U | U | Val119→Leu | |
| 1410 | C | A | A | A | Thr147→Asn | |
| 1584-1585 | CA | AC | AC | AC | Thr205→Asn | |
| 1708 | G | U | U | U | None | |
| 1770 | C | U | Cb | Cb | Ser267→Leu | |
| 1913 | A | G | G | G | Lys315→Glu | |
| NS3 | 4733 | A | G | G | G | Lys46→Glu |
| NS5 | 7712 | A | G | G | G | Asn17→Asp |
| 7801 | A | G | G | G | None | |
a
Results show the nucleotide sequence of E5 (GenBank accession no. AF253420) at the indicated positions in the genome.
b
The underlined sequence change represents the difference between E5-104 and its mouse brain-passaged isolate.
c
Virus isolate 1 was obtained on day 13 from the brain of a moribund mouse that was inoculated i.c. with 10 PFU of E5-104. Virus isolate 2 was harvested on day 12 from the brain of a moribund mouse that was inoculated i.c. with 100 PFU of E5-104.