TABLE 3.
Temperature sensitivity of E5, E5-104, and recombinant E5-651 viruses in Vero and neuroblastoma cells
| Virus | Titer (mean log10 PFU/ml) of virus grown at selected temperatures (°C) in indicated cellsa
|
||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Simian Vero cells
|
Murine Neuro-2A neuroblastoma cells
|
Human SH-SY5Y neuroblastoma cells
|
|||||||||||||||||||
| 32 | 34 | 35 | 36 | 37 | 38 | Δb | 32 | 34 | 35 | 36 | 37 | 38 | Δ | 32 | 34 | 35 | 36 | 37 | 38 | Δ | |
| E5 | 6.7 | 7.2 | 7.4 | 8.3 | 7.4 | 6.8 | −0.7 | 7.2 | 6.7 | 6.3 | 6.6 | 6.2 | 2.7 | 1.0 | 6.1 | 7.1 | 7.3 | 8.0 | 8.0 | 6.7 | 1.9 |
| E5-651 | 6.9 | 6.6 | 7.4 | 7.5 | 7.5 | 6.0 | −0.6 | 7.2 | 7.3 | 7.0 | 6.5 | 5.7 | 3.5 | 1.5 | 7.4 | 7.7 | 7.5 | 7.4 | 7.2 | 6.7 | 0.2 |
| 651/E-Leu119 | 7.5 | 7.1 | 7.2 | 6.4 | 5.2 | 2.6 | 2.3 | 6.8 | 6.2 | 5.5 | 4.9 | <0.7 | <0.7 | >6.1 | 7.9 | 7.5 | 6.7 | 6.4 | 5.7 | 5.0 | 2.2 |
| 651/E-Asn147 | 7.1 | 7.2 | 7.2 | 7.2 | 5.9 | 3.5 | 1.2 | 7.2 | 6.5 | 5.7 | 5.3 | 3.2 | <0.7 | 4.0 | 6.8 | 7.1 | 7.2 | 7.4 | 4.5 | 0.7 | 2.3 |
| 651/E-Asn205 | 7.2 | 7.0 | 6.9 | 6.7 | 5.9 | 3.5 | 1.3 | 5.9 | 6.1 | 5.6 | 5.2 | 3.2 | <0.7 | 3.7 | 7.1 | 7.9 | 7.4 | 6.8 | 6.8 | 2.9 | 0.3 |
| 651/E-Glu315 | 6.1 | 6.0 | 5.6 | 5.4 | 3.7 | 1.0 | 2.4 | 3.7 | 3.0 | 3.1 | <0.7 | <0.7 | <0.7 | >3.0 | 5.4 | 6.1 | 6.4 | 5.4 | 3.1 | <0.7 | 2.3 |
| 651/Ebox | 5.6 | 5.7 | 5.4 | 5.9 | 4.8 | 1.5 | 0.8 | 3.2 | 3.6 | 1.9 | 1.0 | <0.7 | <0.7 | >2.5 | 5.4 | 5.9 | 5.6 | 4.5 | 1.6 | <0.7 | 3.8 |
| 651/NS3-Glu46 | 6.2 | 5.5 | 5.6 | 5.4 | 3.6 | 1.7 | 2.6 | 5.8 | 5.7 | 4.6 | 2.9 | <0.7 | <0.7 | >5.1 | 6.5 | 7.2 | 7.1 | 6.7 | 5.6 | <0.7 | 0.9 |
| E5-104 | 5.7 | 5.2 | 5.5 | 3.4 | <0.7 | <0.7 | >5.0 | 3.6 | 1.0 | <0.7 | <0.7 | <0.7 | <0.7 | >2.9 | 3.1 | 3.9 | 3.4 | 3.5 | <0.7 | <0.7 | >2.4 |
a
Underlined values indicate a reduction of ≥2.0 log10 PFU/ml in virus titer at the indicated temperature compared to that of the titer at the permissive temperature (32°C).
b
The Δ indicates a reduction in mean virus titer (log10 PFU/ml) at 37°C compared to that for the permissive temperature (32°C). Values of ≥2.0 log10 PFU/ml indicate the ts phenotype.