FIG. 4.

(A) Immunoblot of electrophoretically separated lysates from Vero cells mock infected (lane 1) or infected with HSV-1(F) (lane 2), R7041 (lane 3), or R7356 (lane 4) at an MOI of 5. Infected cells were harvested at 12 h postinfection and immunoblotted with anti-UL31 antibody. (B) Caspase 3/7 activity of infected SK-N-SH cells after induction of apoptosis by osmotic shock. SK-N-SH cells were mock infected or infected with HSV-1(F), R7041, or R7356. At 12 h postinfection, the cells were exposed to sorbitol for 1 h, incubated for an additional 5 h, harvested, and assayed for caspase 3/7 activity using a Z-DEVD-aminoluciferin substrate. The values are the means and standard deviations for three independent experiments. Wt, wild type.