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Dependence of US17 expression on viral DNA synthesis. Cells were stained for the presence of US17 and US18 (green), and representative viral immediate-early (IE-2), early (UL44), and late (UL99) gene products (red) 96 h after infection with HCMV(AD169) at an MOI of 0.01 in the absence (−) and presence (+) of PAA (100 μg/ml). Nonconfocal images were obtained at ×20 magnification.
