FIG. 1.

HeLa cells grown on coverslips were infected with herpes simplex virus type 1 (multiplicity of infection of 2), incubated for 10 h, fixed with paraformaldehyde, and immunostained using monoclonal antibodies against nuclear pore complex proteins. Samples were analyzed using a confocal microscope, and images were deconvolved. (A) A mock-infected cell shows a rather regular distribution of nuclear pore complex proteins. (B) Nuclear pore complex proteins are accumulated at the nuclear surface and within the cytoplasm in a herpes simplex virus type 1-infected cell, clearly indicating dramatic changes of nuclear pores.