FIG. 2.

Identification of the role of EBV latent protein EBNA3C in Nm23-H1-dependent modulation of the COX-2 promoter. (A) The 5′-flanking region of COX-2 showing the relative positions of promoter element binding consensus sequences. LUC, luciferase. (B and C) The relative luciferase activity obtained from transcriptional activity of the COX-2 promoter construct in 293T cells. The 90% confluent 293T cells were transfected (+) with 5.0 μg of COX-2 reporter vector pHPES2 (−327/+59) (COX2) with either pA3M-Nm23-H1 (Nm23H1) or pA3M-EBNA3C (EBNA3C) or with both. The results are expressed as the means ± SEMs (error bars) of three separate experiments performed in duplicate or triplicate. Anti-Nm23-H1 (α-Nm23H1) and anti-EBNA3C (α-EBNA3C) antibodies were used.