FIG. 5.

Inhibitory effect of Pin1 is mediated through β-catenin by disruption of its binding to the AR LBD. (A and B) CV1 cells were transfected with pRL-CMV (2.5 ng), ARE₄-luciferase (10 ng), and pCIneo-AR (wild-type AR) (A), or pCIneo-AR(Thr799Ala) (10 ng) (B). Additional plasmids were cotransfected as indicated, and cells were treated with vehicle or DHT (10 nM). −, not used. Results are given in relative light units (RLU). (C) 293T cells were transfected with either 10 μg of pcDNA3.1 vector (−) or pcDNA-Pin1 (+), as indicated. Lysates were precipitated with 5 μg of GST or GST-AR LBD fusion proteins bound to glutathione-agarose beads, and bound β-catenin was determined by immunoblotting.