FIG. 3.

Growth curves of NP31/ATF3-Tet cells (A) and NP31/kinase/ATF3-Tet cells (B) in the presence (ATF3-Off) or absence (ATF3-On) of Tet. Means ± SD of results from four independent experiments are shown. (C) NP31/ATF3-Tet cells (ATF3) or NP31 cells with just the Tet regulation vector (vector) were deprived of Tet for the indicated time before total protein lysates were subjected to anti-CDK4, anti-CDK2, anti-p16, and antiactin Western blotting. A representative blot from three independent experiments is shown.