FIG. 2.
High [K+]i suppresses the SD-induced transcription of Bcl-XS. Effects of SD and TEA on the expression of Bcl-XL/Bcl-XS were assayed by Western blotting (A) and RT-PCR (B). (A) Signal intensities of Bcl-XL/Bcl-XS normalized with actin were means ± standard error of the mean (SEM) of three independent experiments. **, P < 0.01 compared to 0 h. (C) Western blot analysis of effects of H89 or nifedipine on TEA-mediated inhibition of the expression of Bcl-XS induced by SD for 8 h. PDTC (100 μM) blocked the expression of Bcl-XS. (D) Western blot analysis of effects of cesium chloride (Cs; 2 mM) with or without H89 or nifedipine on SD-induced expression of Bcl-XS. H89, 10 μM; nifedipine (Nife), 5 μM. Actin and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) served as loading controls.