FIG. 3.
Down-regulation of p65 blocks the enhanced Bcl-XS expression induced by SD. (A) Transfection efficiency was illustrated by transfecting cultured cortical neurons (from DIV7 to DIV12) with FITC-siRNA with TransMessenger transfection reagent. Western blot analysis showed the efficiency of knockdown of p65 (B) by two different siRNAs for p65 (Sip65-1 and Sip65-2) and the effect of knockdown of p65 on SD-induced Bcl-XS expression (C). SiNS was a negative control for siRNA. The p50 precursor, p105, which exists in cultured rat cortical neurons, served as an unaffected protein to determine the specificity of siRNA-p65. GAPDH served as a loading control.