FIG. 5.

TEA treatment does not affect NF-κB activation or nuclear translocation induced by SD. (A) Time course of activation of NF-κB induced by SD and the effect of TEA on the activation of NF-κB in neurons were analyzed by EMSAs as described in the legend to Fig. 4A. (Right) Data represent means ± SEM of three independent experiments. The asterisks indicate data significantly different from that at 0 h: *, P < 0.05; **, P < 0.01. (B) Effects of SD and TEA for 6 h on IκBα degradation (Western blotting [WB]) and nuclear NF-κB activity (by EMSAs) were illustrated together. Actin served as a loading control. (C) Effects of SD and TEA for 12 h on nuclear p65 level (Nuc) were analyzed by Western blotting. Results from whole lysates of treated neurons demonstrated that the total p65 was not altered (Total). Lamin B and α-tubulin served as loading controls. (D) Confocal images of indirect immunofluorescence of p65 in neurons after different treatments for 12 h.